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Solid Phase Synthesis and Chromatographic Characteristics of Nucleophilic Agents Conjugated with Oligonucleotides Containing 5'-Terminal Carboxyl Group

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Abstract

Conjugates of amines or short peptides with oligonucleotides containing 5'-terminal carboxyl group were prepared by solid phase chemical synthesis. A correlation between the physicochemical parameters and retention times of the synthesized conjugates was established by ion-pair reversed-phase HPLC.

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Authors and Affiliations

  1. Faculty of Chemistry and Belozersky Institute of Physicochemical Biology, Moscow State University, Vorob'evy gory, Moscow, 119992, Russia

    A. V. Kachalova, V. N. Tashlitsky & E. A. Romanova

  2. Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge, CB2 2QH, United Kingdom

    D. A. Stetsenko, M. J. Gait & T. S. Oretskaya

Authors
  1. A. V. Kachalova
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  2. V. N. Tashlitsky
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  3. D. A. Stetsenko
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  4. E. A. Romanova
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  5. M. J. Gait
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  6. T. S. Oretskaya
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Corresponding author

Correspondence to T. S. Oretskaya.

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Kachalova, A.V., Tashlitsky, V.N., Stetsenko, D.A. et al. Solid Phase Synthesis and Chromatographic Characteristics of Nucleophilic Agents Conjugated with Oligonucleotides Containing 5'-Terminal Carboxyl Group. Russian Journal of Bioorganic Chemistry 29, 274–280 (2003). https://doi.org/10.1023/A:1023940617498

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  • DOI: https://doi.org/10.1023/A:1023940617498

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